Studying processes at the distal end of the eukaryotic flagellum/cilium
Flagella and cilia, highly evolutionary conserved organelles, are present on the surface of many eukaryotic cells from protists to mammals. The cilia have critical motility, sensory and signalling roles and their malfunctions in humans lead to a spectrum of diseases called ciliopathies. The distal end of the cilium is important for ciliary beat, signalling, and is the exclusive site of the construction of the ciliary cytoskeleton. Little is known about constituents of the ciliary end hampering our understanding how are the processes occurring in this domain orchestrated. Leveraging the high genetic tractability of the parasitic protist Trypanosoma brucei we identified proteins localizing to the end of its flagellum. We characterize their biochemical properties and infer their cellular roles by studying depletion phenotypes. For a subset of the end-localizing proteins we identified their mammalian orthologs showing that T. brucei is a valuable model to study enigmatic ciliary domains.
Czech Academy of Sciences, Prague, Czech Republic
Domain 1 - UMR 3348 - Genotoxic Stress and Cancer