Quantitative In Situ Imaging of Cellular Lipid Dynamics
Membrane lipids are dynamic molecules that control a wide range of cellular processes, including complex protein-protein interaction and networking, in a spatiotemporally specific manner. To investigate how the local lipid concentration serves as an activation threshold for many cellular processes, we developed a new imaging technology that allows simultaneous in situ quantification of multiple lipids. Application of this technology to various cellular systems led to discovery of new dynamic properties and unusual biological functions of lipids. In this presentation, I will discuss our new and novel findings on the spatiotemporal dynamics and regulatory functions of major classes of mammalian lipids, cholesterol and phosphoinositides.
Department of Chemistry, University of Illinois at Chicago, USA