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Seminar

Friday, November 16th, 2018
From 11h To 12h30
Centre de Recherche - Paris - Amphithéâtre Antoine Lacassagne

Multiplexed mass spectrometric lipid metabolic tracing with single cell sensitivity

  TLipid metabolic tracing is a key technology to understand lipid metabolism in health and disease. We have previously demonstrated tracing with picomole sensitivity and lipid class resolution using alkyne-labeled lipids and detection by fluorogenic click reaction combined with TLC separation. To take advantage of mass spectrometry for analysis, we have developed a family of novel click chemistry MS reporter molecules that combine highly improved sensitivity with enhanced specificity. Introduction of heavy isotopes into the reagents allows multiplexed analysis that saves time and strongly reduces technical noise. The presentation will demonstrate the performance of the system in tracing hepatic lipid metabolism in more than ten lipid classes with several hundreds labeled lipid species. Finally, I will demonstrate how the femtomole sensitivity of the method can be used to trace fatty acid incorporation in single hepatocytes.

Speaker(s)

Dr. Christoph Thiele

Rheinische Friedrich-Wilhelms-Universität, Bonn, Allemagne

Hosted by

Dr. Ludger Johannes

Institut Curie

Invited by

Dr. Ludger Johannes

Institut Curie