> Retour à la liste des séminaires

Séminaire

Vendredi 18 Mai 2018
De 11h à 12h
Centre de Recherche - Paris - Amphithéâtre Biologie du développement et cancer

Les Bourgeons du Mal: Membrane budding and severing by the ESCRT complexes

Budding and release from the plasma membrane of the producer cell are the penultimate steps in the HIV replication cycle. The talk is entitled "Les Bourgeons du Mal" because of the essential role of the budding step in the production of this lethal pathogen, which has killed 35 million people globally and is thought to infect another 38 million worldwide. Budding of the virus is driven by the viral Gag protein, while virion release requires the host ESCRT machinery. The ESCRT machinery has many functions in normal physiology, from endosomal sorting to cytokinesis, and beyond. My laboratory spent most of the 2000s working on the structures of the ESCRT proteins and their interactions with HIV Gag, and more recently we have focused on mechanistic reconstitution of the ESCRTs alone and as they function with Gag. I will describe highlights of the core structural organization of the ESCRTs, along with their structural and biochemical mechanisms of recruitment to HIV budding sites.  I will explain how ESCRTs interact with negatively curved membranes. The unique property of the ESCRTs is to carry out "reverse topology" membrane scission, that is, to cut narrow membrane necks whose interior is contiguous with the cytosol. Various models for membrane scission will be described. I will end by presenting our lab's reconstitution of ESCRT membrane scission using membrane nanotubes pulled from giant unilamellar vesicles (GUVs).

Orateur(s)

Pr Jim Hurley
Prof

California Institute for Quantitative Biosciences, University of California, Berkeley

Invité(e)(s) par

Patricia Bassereau
Directeur de Recherche

Institut Curie

Bruno Goud
Directeur de Recherche

Institut Curie

Contact

Patricia Bassereau

Directeur de Recherche

Institut Curie

Lui envoyer un mail

Bruno Goud

Directeur de Recherche

Institut Curie

Lui envoyer un mail

En bref

Recent papers:

- Gardner B. M., Castanzo D. T., Chowdhury S., Stjepanovic G., Stefely M. S., Hurley J. H., Lander G. C., Martin A. The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading Nat. Commun. 9 135 (2018)

- Schoneberg J., Lee I. H., Iwasa J. H., Hurley J. H. Reverse-topology membrane scission by the ESCRT proteins Nat. Rev. Mol. Cell Biol. 18 5-17 (2017)

- Young L. N., Cho K., Lawrence R., Zoncu R., Hurley J. H. Dynamics and architecture of the NRBF2-containing phosphatidylinositol 3-kinase complex I of autophagy Proc Natl Acad Sci U S A 113 8224-8229 (2016)

- Schulze-Gahmen U., Echeverria I., Stjepanovic G., Bai Y., Lu H., Schneidman-Duhovny D., Doudna J. A., Zhou Q., Sali A., Hurley J. H. Insights into HIV-1 proviral transcription from integrative structure and dynamics of the Tat:AFF4:P-TEFb:TAR complex Elife 5 (2016)

- Carlson L. A., Bai Y., Keane S. C., Doudna J. A., Hurley J. H. Reconstitution of selective HIV-1 RNA packaging in vitro by membrane-bound Gag assemblies Elife 5 (2016)

- Lee I. H., Kai H., Carlson L. A., Groves J. T., Hurley J. H. Negative membrane curvature catalyzes nucleation of endosomal sorting complex required for transport (ESCRT)-III assembly Proc Natl Acad Sci U S A 112 15892-15897 (2015)