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Séminaire

Mardi 24 Novembre 2020
De 9h30 à 11h
Centre de recherche - Paris - Amphithéâtre Marie Curie

Real-time visualization of drug resistance acquisition by horizontal gene transfer reveals a new role for AcrAB-TolC multidrug efflux pump

© Institut Curie

Drug resistance dissemination by horizontal gene transfer remains poorly understood at the cellular scale. Using live-cell microscopy, we reveal the dynamics of resistance acquisition by transfer of the F conjugative plasmid encoding the tetracycline-efflux pump TetA. We show that the entry of the ssDNA plasmid into the recipient cell is rapidly followed by complementary strand synthesis and expression of newly acquired genes. TetA production is enhanced by zygotic induction resulting in the optimized establishment of resistance. In the presence of protein synthesis-inhibiting antibiotics, acquisition of resistance becomes strictly dependent on AcrAB-TolC multidrug efflux pump. We demonstrate that AcrAB-TolC is required for maintenance of protein synthesis activity and therefore TetA production following plasmid acquisition. This work uncovers a novel essential role of multidrug efflux systems in the acquisition of drug-specific resistance by horizontal gene transfer and helps to understand the dissemination of antibiotic resistance in bacteria.

Orateur(s)

Dr Christian Lesterlin
Université de Lyon

Institut de Biologie et Chimie des Protéines

Organisateur(s)

Mme Caroline Audouin

Institut Curie

Invité(e)(s) par

Dr Miné-Hattab Judith

Institut Curie

Contact

Mme Caroline Audouin

Institut Curie

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