Novel roles of the centrosome protein ninein in mouse development
We have generated mice in which the gene of the microtubule-organizing protein ninein was knocked out constitutively, and specifically in the epidermis. Both types of mice showed epidermal defects during embryonic development, leading to a compromised skin barrier. At the cellular level, keratinocytes in ninein-null epidermis failed to reorgainze microtubules into a differentiation-specific cortical network. Accompanying defects in desmosome assembly and in the secretion of lamellar bodies were seen. Moreover, the constitutive knockout caused additional developmental defects, involving retarded growth, and skeletal abnormalities. We are trying to determine what cellular defects are responsible for the defects in mouse development.
DR, directeur de recherche, CNRS/Université Paul Sabatier, Centre de Biologie du Développement, Toulouse
Domain 4 - UMR 144 - Cell Biology and Cancer