Hematopoietic stem cell fate decisions, clonal hematopoiesis, and clinical implications & Basics of Cellular Barcoding
Prof. Dr. Michael RIEGER
“Hematopoietic stem cell fate decisions, clonal hematopoiesis, and clinical implications”
Life-long blood regeneration relies on hematopoietic stem cell (HSC) maintenance, integrity and renewal. However, despite of the importance of a balanced and protected HSC pool, a continuous and adequate differentiation into all blood cell lineages, dependent on the demand, is equally important. Both stem cell fate decisions, self-renewal and differentiation, are orchestrated by extrinsic signals and intrinsic molecular programs.
We are interested in the molecular control of these early cell fate decisions, how extrinsic cues are integrated in the maintenance of stable HSC decision programs, and how these programs are altered in malignancy. Long-term time-lapse microscopy-based cell tracking combined with molecular studies and in vivo examinations allow us to link molecular control with future cell fate.
The incidence of clonal hematopoiesis (CH) caused by somatic mutations in HSCs is certainly not rare in elderly persons without hematologic abnormalities. These mutations alter HSC fate decisions and mature blood cell function, and lead to clonal dominance. Most intriguingly, clonal hematopoiesis is associated with cardiovascular diseases, and we investigate the clinical and functional association of CH in chronic heart failure and other cardiovascular diseases.
Dr. Leonid BYSTRYKH ERIBA
"Basics of Cellular Barcoding"
The cellular barcoding method, although not entirely new, is on the rise of popularity in recent literature. The dream of unique labelling every cell in the multicellular environment persist for at least a decade. There are numerous attempts to demonstrate success in this field. The major technique used at present is DNA barcoding, which uses some available tools to deliver unique DNA short sequence into the genome.
It is usual that authors discuss the end results of such experiments and share the most colorful and exciting outcomes. In my talk I will focus on the most boring technical part of this story. Instead of talking about exciting results I will focus on the methodological part. The attention will be given to the definition of counts, principles and concepts in construction of the library, essential features of the barcodes, reading barcodes from sequencing data, quantification of the clonal output.
LOEWE Centre for Cell and Gene Therapy, Frankfurt - Germany
European Research Institut for the Biology of Aging - The Netherlands