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Wednesday, June 19th, 2019
Centre de Recherche - Paris - Amphith√©√Ętre Marie Curie

Epigenetic programming of macrophage differentiation and glucocorticoid responses

The molecular basis of cellular memory is a current knowledge frontier. In the immune system, cellular memory likely extends beyond cell fate determination mechanisms since immunity can tailor its responses to a potentially hostile environment that is a priori variable if not unpredictable. One particularly versatile innate immunity cell type is the macrophage (Mf), which can be derived in vitro from circulating blood monocytes. These cells can adopt different polarisation states depending on external factors such as microbe-associated molecular patterns. We extensively profiled the ‘trained’ Mf state elicited by beta-glucans, that model vaccine adjuvant effects, and an LPS-induced ‘tolerized’ Mf state that models bacterial sepsis. Also, the impact of Mf polarisation on glucocorticoid responses was studied and found to differ substantially, providing clear evidence for cell-state modulation of the glucocorticoid response. To integrate epigenomic and transcriptomic data sets, we explored the use of HiC-based topologically associating domain (TAD), specifically asking whether they do confine epigenetic and transcriptomic signalling. The results we obtained support the notion that TADs naturally integrate signalling to the human genome.


Prof. Colin Logie
Associate Professor Faculty of Science

Radboud Institute for Molecular Life Sciences

Hosted by

Invited by

Genevieve Almouzni
Domain 2 - UMR 3664 - Nuclear Dynamics

Institut Curie

Jean-Pierre Quivy
Domain 2 - UMR 3664 - Nuclear Dynamics

Institut Curie