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Seminar

Thursday, February 21st, 2019
14h
Centre de Recherche - Paris - Amphith√©√Ętre Antoine Lacassagne

Dynamics of immune cell migration and interaction visualized by intravital microscopy

During the last decade, intravital optical microscopy has launched a new trend in the field of biology. By using this advanced imaging technique we have established a new system for visualizing in situ behavior of a diversity of living cells within intact tissues and organs. Among them, we succeeded in visualizing the various dynamic phenomena within bones and joints, where various kinds of immune cells are produced and functioning although poorly analyzed by conventional methodology such as histological analyses with decalcified sections. We have so far identified the real modes of migration, differentiation and function of bone-destroying osteoclasts, special kind of macrophages responsible for bone and joint erosions. In addition to the application to animal experimental models, we are currently trying to adapt this technique for evaluating disease status in local foci of human patients. In this presentation I will present the recent update on intravital imaging studies on immune and other systems for clarifying in vivo behaviors of cell and tissue dynamics.

Speaker(s)

Masaru Ishii

Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka, Japan

Invited by

Eliane Piaggio
Domain 3 - U932 - Immunity and Cancer

Institut Curie

Ana-Maria Lennon
Domain 3 - U932 - Immunity and Cancer

Institut Curie

Contact

Eliane Piaggio

Institut Curie

Ana-Maria Lennon

Institut Curie

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Publications:

 

Furuya M, et al. Direct cell-cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo. Nat. Commun., 9(1):300, 2018.

 

Matsuura Y, et al. In vivo visualization of different modes of action of biologic DMARDs inhibiting osteoclastic bone resorption. Ann. Rheum. Dis., 77(8):1219-1225, 2018.

 

Maeda H, et al. Real-time intravital imaging of pH variation associated with cell osteoclast activity and motility using designed small molecular probe. Nat Chem Biol., 12(8):579-85, 2016.

 

Nishikawa K, et al. Dnmt3a regulates osteoclast differentiation by coupling to an S-adenosyl methionine-producing metabolic pathway. Nat. Med., 21(3):281-7, 2015.