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Séminaire

Mardi 20 Novembre 2018
De 11h30 à 13h
Centre de Recherche - Paris - Amphithéâtre Marie Curie

Plasmid DNA transfection, revealing tolerant yet separatist mammalian cells

The genome of mammalian cells is challenged by extra-chromosomal DNA introduced by viruses, bacteria, or transfection of plasmid DNA. However, how cells handle these DNA molecules is largely unknown.

We analyzed predominantly Hela cells stably expressing fluorescently tagged LacI protein and transfected with plasmid DNA containing LacO repeats. We found thousands of transfected plasmids in one cytoplasmic cluster being asymmetrically propagated over several divisions. I will present the principles underlying the biased propagation of the plasmid cluster to the cell with the young centrosome and the characteristics of this DNA cluster. Our data suggest that cytoplasmic DNA is maintained in the cytosol of mammalian cells by its enclosing special double membrane that contains LEM-domain proteins and that this cytoplasmic compartment of DNA might act as collector of extra-chromosomal DNA.

Orateur(s)

Dr Ruth KROSCHEWSKI
PhD

ETH Zurich - Institute of Biochemistry, Zurich

Invité(e)(s) par

Dr Renata BASTO
Domain 4 - UMR 144 - Subcellular Structure and Cellular Dynamics

Institut Curie

Contact

Dr Renata BASTO

Institut Curie

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