Imprinted loci as a model for studying epigenetic regulation of alternative polyadenylation
The transcriptome of a cell is tightly regulated by epigenetic mechanisms including DNA methylation and histone modifications. We previously found that epigenetic mechanisms can influence the utilization of alternative polyadenylation sites and induce intron retention. Others have shown that epigenetic factors influence alternative splicing. We have exploited an imprinted locus to examine the contribution of epigenetic factors to RNA processing in detail. While CpG islands (CGIs) are generally associated with the transcription start sites of genes, we have examined intragenic CpG islands (iCGIs) located in gene bodies because a major role of iCGIs is linked to tissue-specific gene expression where tight control over transcription during development is crucial. Using a broader approach and examining the entire mammalian genome in two species, we found that DNA methylation and histone modifications work in a tissue-specific context to influence poly(A) site choice and generate transcriptome diversity.
Professor of Epigenetics | Dean for Doctoral Studies | King's College London, UK
Domain 1 - UMR 3348 - Genotoxic Stress and Cancer
Domain 2 - UMR 3215 / U934 - Genetics and Developmental Biology