Dynamics of epithelial cells during collective migration and cell extrusion
The directed migration of cell collectives is essential in various physiological processes, such as epiboly, drosophila dorsal closure and convergent extension during morphogenesis as well as pathological events like wound healing and cancer metastasis. Collective cell migration leads to the emergence of coordinated movements over multiple cells. In the first part, we will show that the establishment of single-cell level polarity could drive coordinated movements of epithelial cells. We designed a minimalistic model system to generate one-dimensional epithelial trains confined in ring-shaped patterns, permitting simplified biomechanical analysis of collective cell migration. Our approaches show that the maintenance of coordinated migration relies on the acquisition of a front-back polarity of each single cell but not on cell-cell junctions. In the second part, we will show how epithelial movements and stresses drive cell extrusion mechanisms from epithelial tissues.
Co-Principal Investigator, Mechanobiology Institute, National University of Singapor
Institut Jacques Monod (Université Paris Diderot and CNRS), Mechanobiology Institute (Singapore, NUS)
Domain 4 - UMR 144 - Subcellular Structure and Cellular Dynamics