Image Reconstruction and Application to Whole-Organism Transcription Imaging
Modern developmental biology increasingly relies on imaging of large samples with high spatial and temporal resolution. Scientists can acquire high-resolution images of large living and fixed samples by extending the field-of-view of high-resolution optical microscopes using multi-tile or multi-view acquisition modes on wide field, confocal or light sheet microscopes. We present software to interactively view and reconstruct such terabyte-sized acquisitions of developing organisms and entire cleared samples such as mouse brains or mouse embryos.
We will illustrate how we apply these powerful imaging tools to study transcriptional regulation underlying C. elegans development. We will present approaches that combine single-molecule fluorescent in-situ hybridization to detect individual mRNA molecules with lightsheet and electron microscopy acquisitions of entire C. elegans specimen. We will highlight two current projects in the lab focusing on the molecular mechanisms of sex determination and the choice of the alternative larval dauer stage geared for survival. Our multi-modal, model-based approaches have the potential to decipher molecular mechanisms underlying important developmental events that ensure proper development of organisms.
Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC)
Domain 2 - UMR 3215 / U934 - Genetics and Developmental Biology